Journal: Molecular therapy. Nucleic acids

Article Title: Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles.

doi: 10.1016/j.omtn.2023.03.002

Figure Lengend Snippet: Figure 5. CCL2-CCR4 axis regulates plucking-induced hair regeneration (A) F4/80 immunostaining and statistics show recruitment of M1 macrophage at PPD3 after plucking. (B) CD206 immunostaining and statistics show no obvious recruitment of M2 macrophage at PPD3 after plucking. (C) TNF-a immunostaining and statistics show skin inflammation at PPD3 after plucking. (D) K14 immunostaining and statistics show hair regenerate at PPD3 after plucking. (E) K14 immunostaining and statistics show hair regeneration is blocked after inhibition of CCL2 (iCCL2) or CCR4 (iCCR4) at PPD3. (F) F4/80 immunostaining and statistics show recruitment of M1 macrophage is disrupted after inhibition of CCL2 (iCCL2) or CCR4 (iCCR4) at PPD3. (G) CD206 immunostaining and statistics show no obvious recruitment of M2 macrophage at PPD3 in all three groups. (H) TNF-a immunostaining and statistics show significantly decreased skin inflammation after inhibition of iCCL2 or iCCR4 at PPD3. *p < 0.05, **p < 0.01, ***p < 0.001, #, no significance, n R 3.

Article Snippet: Primary antibodies include AE13 (ab16113, Abcam), AE15 (gift), CD34 (ab81289, Abcam), Sox9 (ab71762, Abcam), K10 (ab76318, Abcam), K14 (A01432, Boster), CD86 (ab53004, Abcam), F4-80 (sc-25830, Santa Cruz), CD163(sc-33560, Santa Cruz), CD206(sc58986, Santa Cruz), TNF-a (AF8208, Beyotime), NCAM (from Dr. Cheng-Ming Chuong laboratory), P16 (ab51243, Abcam), P63 (12143-1-AP, Proteintech), P-cadherin (13-2000Z, Thermo), and PCNA (ab92552, Abcam).

Techniques: Immunostaining, Inhibition

Journal:

Article Title: Differential Expression of Cytokeratin after Orthotopic Implantation of Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability

doi:

Figure Lengend Snippet: Range of Monoclonal Antibodies Used for Western Blotting

Article Snippet: Source K8.12 1 :100 CK 13, 16 13 BioMakor AE-1 1 :100 CK 14, 16, 19 7 PROGEN (most type I CK) 6B10 1 :100 CK 4 14 SIGMA Ks6.KA12 1 :100 CK 6 15 PROGEN RCK105 1 :5 CK 7 16 PROGEN M20 1 :10 CK 8 17 PROGEN KS-1A3 1 :100 CK 13 18 SIGMA LL002 1 :100 CK 14 19 CYMBUS BIOTEC Ks18.04 1 :20 CK 18 1 PROGEN K4.62 1 :100 CK 19 20 BioMakor CCI610 1 :100 vimentin 21 YLEM Open in a separate window Range of Monoclonal Antibodies Used for Western Blotting

Techniques: Western Blot

Journal: PeerJ

Article Title: A gelatin/collagen/polycaprolactone scaffold for skin regeneration

doi: 10.7717/peerj.6358

Figure Lengend Snippet: CK-14 was a specific protein for PHEK, whereas alpha tubulin was a specific protein for PHDF. w/o, without. (scale bar: 250 µm).

Article Snippet: PHEK on G∕C∕P biocomposites (1:8 and 1:20) at 1 and 3 days were labeled with the (primary) monoclonal rabbit anti-human cytokeratin (CK)-14 1:100 (Bioworld Technology), and the (secondary) rhodamine-conjugated polyclonal goat anti-rabbit IgG 1:200 (Chemicon, Billerica, Mass) examined under a fluorescent microscope.

Techniques:

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Sources, dilutions, fixations and pretreatments of the antibodies used

Article Snippet: CK 14 , Cytoskeleton, cell differentiation , LL002 , Mouse , Leica Biosystems, Newcastle, UK , 1:100 , m , Tris‐EDTA buffer pH 9.0, 20 min steamer.

Techniques: Cell Differentiation, Marker

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Semiquantitative analysis of immunohistochemical staining for cytoskeletal proteins, proliferation, differentiation, and stem cell markers. CK 14, p63, and NGF showed a gradual decrease from crypt to center. Statistically significant age differences (*, P < 0.05) were detected in the limbal zone for NGF and in the noncrypt zone for ABCG2. Bars represent mean values from six horses of both age groups and whiskers represent SD.

Article Snippet: CK 14 , Cytoskeleton, cell differentiation , LL002 , Mouse , Leica Biosystems, Newcastle, UK , 1:100 , m , Tris‐EDTA buffer pH 9.0, 20 min steamer.

Techniques: Immunohistochemical staining, Staining

Journal: Veterinary Ophthalmology

Article Title: Morphological and immunohistochemical characteristics of the equine corneal epithelium

doi: 10.1111/vop.12651

Figure Lengend Snippet: Immunohistochemical staining of intermediate filaments CK 14, CK 19, CK 3, CK 10, and vimentin. Representative pictures of crypts, limbus, and central corneal epithelium (and skin used as positive control for CK 10, see inserts) are shown for both foals (left panels) and adult horses (right panels). Potential stem cell marker CK 14 was present in every examined region with decreasing frequency toward the center. Cornea‐specific differentiation marker CK 3 was generally present throughout the superficial and intermediate layer, absent in the most basal layer of crypt and limbal epithelium and showed decreased staining intensity in the basal layer of the peripheral and central corneal epithelium. Scale bar =30 µm

Article Snippet: CK 14 , Cytoskeleton, cell differentiation , LL002 , Mouse , Leica Biosystems, Newcastle, UK , 1:100 , m , Tris‐EDTA buffer pH 9.0, 20 min steamer.

Techniques: Immunohistochemical staining, Staining, Positive Control, Marker